Cost to paint 2 car garage floor9/3/2023 ![]() Keep in mind that the sidewalls will not get any foot traffic and are only cosmetic. We were able to paint the sidewalls and it dried with no issues. So we sealed the bag and the epoxy paint was still fine the next morning. Because the floor was wet we had to wait until the next day before we could paint the side walls. We initially didn’t plan on using the epoxy paint on the 4” sidewalls of our garage, but we had enough paint leftover that we painted the sidewalls too. Not only did we have enough to safely cover the 21 x 23 garage floor, but we also had some leftover. I felt they were fairly generous in their estimate. Add the cost of the kit to that and you’re looking at around $1000 to have someone come in and do the job for you. For example, if you spend $200 on your floor paint, multiply that by four to get the rough labor cost. Using that logic I would estimate multiplying the cost of the material by 4 for a more realistic cost estimate for this job. To prepare the surface for painting you can expect to go over the floor surface at least 4 times to properly clean and prepare it for painting. Surface preparation plays a huge role in the success of this project. Then after completely drying, a return trip for painting is required. It typically requires the better part of a day for cleaning and preparation. However, applying epoxy floor paint is not a simple process of rolling the paint on and you’re done. That should get you in the ballpark for most projects. Just be ready to reach deep in your pocketbook! In our experience over the years, for most home projects, we have found that you can take the cost of the materials for the job and roughly double it. ![]() You can always hire someone to do the job. Not sure you want to take on painting your garage floors.
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Engorged tick9/3/2023 ![]() Unfortunately, if your dog likes to spend time outdoors, there’s no way to completely avoid contact with ticks, but you can help protect against ticks by checking your dog daily and using a proven tick treatment like NexGard ® or NexGard SPECTRA ®. Given the potentially life-threatening diseases they can cause, it is only natural to want to learn about how to avoid ticks on dogs. Take care not to squeeze the tick’s body. Using tweezers or a specially-designed tick removal tool, grab the tick adjacent to your dog’s skin (at the tick mouthparts), twist the tick, then pluck the tick away from the skin. You may be concerned about how best to remove larger, engorged ticks from their dog, but the process is similar for ticks of all sizes. How to Remove an Engorged Tick from a DogĪ tick will become engorged once it has fed and become swollen with blood. Ticks can be placed in alcohol after removal to kill them. It is important to remove ticks as soon as possible with tweezers or a specially-designed tick removal tool. Similar to the use of Vaseline, the use of alcohol to kill ticks on dogs is not recommended. How to Remove a Tick from a Dog with Alcohol Using Vaseline to kill ticks on dogs is not recommended. How to Remove a Tick from a Dog with Vaseline ![]() If you have any concerns about your dog’s health, you should seek immediate veterinary attention.
Sysco invoices online9/2/2023 ![]() The physical, mental, and environmental conditions in which the work is performed. Proficient use of MS Windows and Office (Word, Excel, PowerPoint, Access and Outlook) and familiarity in maintaining a customer database and Internet navigation.Detail Oriented – Attention to details and accuracy.Professionalism - Approaches others in a tactful manner.Judgment - Displays willingness to make decisions.Gathers and analyzes information skillfully. Problem Solving - Identifies and resolves problems in a timely manner.Planning / Organization - Ability to prioritize and manage multiple priorities/deadlines and shift priorities as necessary.Customer Service – Respond promptly to requests for service and assistance as needed.Capable of working with internal staff from other departments in a proactive and constructive manner. Ability to effectively present information and respond to questions from suppliers, customers, management and inter-department staff. Excellent communication (verbal and written), interpersonal and professional interactive skills are necessary to perform at a high degree of proficiency.Skills & Abilities (Years & Type of Experience): 2 years’ Customer Service experience, including working knowledge of shipping procedures and practices inventory control processes, procedures and practices and inventoried product line specifications (dimensions, weight, etc.).Minimum Experience (Years & Type of Experience): Minimum Education, Including Degrees & Certifications: ![]() Purchasing – tracking and proof of delivery, ETA on drop shipments, items discontinued for delivery replacement items Accounting – update on orders for Release on Hold. Peptide backbone of a protein9/2/2023 The main body of research on CPPs focuses on synthetic peptides (54.8%) based on L-amino acids (84.3%) ( Figure 1c). In addition, proteins (9%), nanoparticles (7.8%) and peptide therapeutics (4.7%) are other cargoes of interest ( Figure 1b). The major cargo of biomedical relevance is nucleic acids (16%). Interestingly, the statistics section of the CPP database site ( Figure 1) reveals that there are 1699 unique CPP sequences and that most are linear CPPs (94.5%) ( Figure 1a) used for the delivery of fluorophores (58.4%) ( Figure 1b). Ī CPP database, established in 2012 and now updated to CPP version 2.0, is a useful source of information. Positively and negatively charged, amphipathic (primary or secondary) and non-amphipathic CPPs are used today in in vitro and in vivo studies. Inspired by these molecules and focused mainly on positively charged sequences, many CPPs with differences in charge, polarity and/or structure have been discovered and/or designed. Discovered in the 1990s, the first reported examples of CPPs were the Tat peptide (positions 48–60, derived from the transcription protein of HIV-1) and penetratin (derived from the amphiphilic Drosophila Antennapedia homeodomain). An alternative terminology, the protein transduction domain (PTD), was used initially to describe such peptides as the first examples of CPPs were based on natural peptides derived from protein fragments. Therefore, the transition of CPPs to a clinical setting remains a challenge also due to the concomitant involvement of various internalization routes and heterogeneity of cells used in the in vitro studies.Ĭell-penetrating peptides (CPPs) are short peptides (<30 amino acids long) that are able to penetrate biological membranes and drive the internalization of a bioactive cargo in cells. This led to increased design complexity of new CPPs that does not always result in greater CPP activity. The application of distinct design strategies to known physico-chemical properties of CPPs offers the opportunity to improve their penetration efficiency and/or internalization kinetics. In this review, a variety of CPP designs will be described, including linear and flexible, positively charged and often amphipathic CPPs, and more rigid versions comprising cyclic, stapled, or dimeric and/or multivalent, self-assembled peptides or peptido-mimetics. Following these nature-inspired examples, a number of rationally designed CPPs has been developed. The discovery of cell-penetrating peptides (CPPs) and the early design approaches through mimicking the natural penetration domains used by viruses have led to greater efficiency of intracellular delivery. ![]() Thus, compounds that efficiently cross cell membranes are the key to improving the therapeutic value and on-target specificity of non-permeable drugs. Efficient intracellular drug delivery and target specificity are often hampered by the presence of biological barriers. My bitch a choosy lover9/2/2023 ![]() Need a real street stalker (stalker) to walk a green mile When I say my girl I don't mean my woman, that ain't my style I show you shit you never seen, the Seven Wonders of the WorldĪnd I can make you the eighth if you wanna be my girl The grass is greener on that other side, if you know what I mean Your bitch chose me, you ain't a pimp, you a fairyīaby you been rollin' solo, time to get down with the team Some hoes wanna choose but them bitches too scary I smashed up the gray one, bought me a redĮvery time we hit the parkin' lot we turn head She be cross country, givin' all that she gotĪ thousand a pop, I'm pullin' Bentleys off the lot ![]() Top notch hoes get the most, not the lesserįuckin' up the game, bitch it gets no love Never in the sheets, like it on top of the cover My bitch a choosy lover, never fuck without a rubber Man these girls is smart, Three Stacks, these girls is smart Hey keep your heart, Three Stacks, keep your heart Keep your heart, Three Stacks, keep your heart I know you ain't a pimp, but pimp remember what I taught ya: If that bitch do you dirty we'll wipe her ass out in some detergent You know we got your back like chiroprac-tic Like a preemie out the womb, my partner yelling "too soonĭon't do it! Reconsider, read some liter. When I shoot the moon, high jump the broom So the light from the sun would not burn me on my bum They dip as quick as they can, the atmosphere is now ripped Spaceships don't come equipped with rear view mirrors ![]() Give up all this pussy cat that's in my lap, no lookin' back Peninsula maybe, it makes no sense, I know crazy Wetness all around me, true but I'm no island Then I CC'ed every girl that I'd see-seed 'round townĪnd hate to see y'all frown but I'd rather see her smilin' Sayin' that I chose this cutie pie with whom I wanna beĪnd I apologize if this message gets you down So I typed a text to a girl I used to see Brave 2012 merida9/2/2023 ![]() Young Merida: AHH!!!! Fergus: Mor'du?! Elinor, run!įergus: Then out of nowhere, the biggest bear you've ever seen! Its hide littered with the weapons of fallen warriors! Its face scarred with one dead eye. Why not use your very own? Happy birthday, my wee darlin'! Where is my little birthday girl, hm? I'm going to gobble her up when I find her! I'm going to eat you! Ach! Fergus, no weapons on the table! Young Merida: Can I shoot an arrow? Can I, can I, can I, can I? Please, can I? King Fergus: Not with that. Mend the bond torn by pride.ĭialogue Queen Elinor: Where are you? Come out! Come out! Come on out! I'm coming to get you! Where are you, you little rascal? I'm coming to get you! Hmm. You never conjure where you carve very important.You only have to be brave enough to see it. That destiny is not our own, but I know better. There are those who say fate is something beyond our command.But when we were threatened from the north, you joined together to defend our lands. Our kingdom is young our stories are not yet legends, but in them, our bond was struck. Where did you get this water? It has worms! Find those by the creek, did you? They're nightshade berries.I didn't ask her to change you into a bear! I just wanted her to change.you. Mum? You're.You're a BEAR! Why a bear? OH! That scaffy witch gave me a gammy spell! It's not my fault.But every once in a while, there's a day when I don't have to be a princess.They get away with murder! I can never get away with anything! I became a sister to three new brothers. ![]() It's the one thing we search for, or fight to change. Others say fate is woven together like a cloth, so that one's destiny intertwines with many others. Some say our destiny is tied to the land, as much a part of us as we are of it. AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |